AustralieFrV1, Main, Exploration, bibRecord, 00B164

Identification of slow and fast-acting toxins in a highly ciguatoxic barracuda (Sphyraena barracuda) by HPLC/MS and radiolabelled ligand binding

Identifieur interne : 00B164 ( Main/Exploration ); précédent : 00B163; suivant : 00B165

Identification of slow and fast-acting toxins in a highly ciguatoxic barracuda (Sphyraena barracuda) by HPLC/MS and radiolabelled ligand binding

Auteurs : Ivannah Pottier [France] ; Brett Hamilton [Australie] ; Alun Jones [Australie] ; Richard J. Lewis [Australie] ; Jean Paul Vernoux [France]

Source :

Toxicon : (Oxford) [ 0041-0101 ] ; 2003.

RBID : Pascal:04-0052439

Descripteurs français

Pascal (Inist)
- Identification, Spectrométrie masse, Toxine, Ciguatera, Contamination biologique, Poisson comestible, Aliment, Animal, Souris, Essai biologique, Extrait, Voie intrapéritonéale, Ciguatoxine, Dinophyta, Guadeloupe, Homme, Intoxication alimentaire, Toxicité, Chromatographie HPLC, Brevetoxine.
Wicri :
- geographic : Guadeloupe.
- topic : Homme, Intoxication alimentaire.

English descriptors

KwdEn :
- Animal, Bioassay, Biological contamination, Ciguatera, Dinophyta, Edible fish, Extract, Food, Food poisoning, Guadeloupe, HPLC chromatography, Human, Identification, Intraperitoneal administration, Mass spectrometry, Mouse, Toxicity, Toxin.

Abstract

A barracuda implicated in ciguatera fish poisoning in Guadeloupe was estimated to have an overall flesh toxicity of 15 MUg/g using mouse bioassay. A lipid soluble extract was separated into two toxic fractions, FrA and FrB, on a LH20 Sephadex column eluted with dichloromethane/methanol (1:1). When intraperitoneal injected into mice, FrA provoked symptoms characteristic of slow-acting ciguatoxins, whereas FrB produced symptoms indicative of fast-acting toxins (FAT). High performance liquid chromatography/mass spectrometry/radio-ligand binding (HPLC/h4S/RLB) analysis confirmed the two fractions were distinct, because only a weak overlap of some compounds was observed. HPLC/MS/RLB analysis revealed C-CTX-1 as the potent toxin present in FrA, and two coeluting active compounds at m/z 809.43 and 857.42 in FrB, all displaying the characteristic pattern of ion formation for hydroxy-polyethers. Other C-CTX congeners and putative hydroxy-polyether-like compounds were detected in both fractions, however, the RLB found them inactive. C-CTX-1 accounted for >90% of total toxicity in this barracuda and was confirmed to be a competitive inhibitor of brevetoxin binding to voltage-sensitive sodium channels (VSSCs) with a potency two-times lower than P-CTX-1. However, FAT active on VSSCs and <900 Da were suspected to contribute to the overall toxicity.

Affiliations:

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Le document en format XML

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<s3>FRA</s3>
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<sZ>5 aut.</sZ>
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<wicri:noRegion>14032</wicri:noRegion>
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<series><title level="j" type="main">Toxicon : (Oxford)</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animal</term>
<term>Bioassay</term>
<term>Biological contamination</term>
<term>Ciguatera</term>
<term>Dinophyta</term>
<term>Edible fish</term>
<term>Extract</term>
<term>Food</term>
<term>Food poisoning</term>
<term>Guadeloupe</term>
<term>HPLC chromatography</term>
<term>Human</term>
<term>Identification</term>
<term>Intraperitoneal administration</term>
<term>Mass spectrometry</term>
<term>Mouse</term>
<term>Toxicity</term>
<term>Toxin</term>
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<keywords scheme="Pascal" xml:lang="fr"><term>Identification</term>
<term>Spectrométrie masse</term>
<term>Toxine</term>
<term>Ciguatera</term>
<term>Contamination biologique</term>
<term>Poisson comestible</term>
<term>Aliment</term>
<term>Animal</term>
<term>Souris</term>
<term>Essai biologique</term>
<term>Extrait</term>
<term>Voie intrapéritonéale</term>
<term>Ciguatoxine</term>
<term>Dinophyta</term>
<term>Guadeloupe</term>
<term>Homme</term>
<term>Intoxication alimentaire</term>
<term>Toxicité</term>
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<front><div type="abstract" xml:lang="en">A barracuda implicated in ciguatera fish poisoning in Guadeloupe was estimated to have an overall flesh toxicity of 15 MUg/g using mouse bioassay. A lipid soluble extract was separated into two toxic fractions, FrA and FrB, on a LH20 Sephadex column eluted with dichloromethane/methanol (1:1). When intraperitoneal injected into mice, FrA provoked symptoms characteristic of slow-acting ciguatoxins, whereas FrB produced symptoms indicative of fast-acting toxins (FAT). High performance liquid chromatography/mass spectrometry/radio-ligand binding (HPLC/h4S/RLB) analysis confirmed the two fractions were distinct, because only a weak overlap of some compounds was observed. HPLC/MS/RLB analysis revealed C-CTX-1 as the potent toxin present in FrA, and two coeluting active compounds at m/z 809.43 and 857.42 in FrB, all displaying the characteristic pattern of ion formation for hydroxy-polyethers. Other C-CTX congeners and putative hydroxy-polyether-like compounds were detected in both fractions, however, the RLB found them inactive. C-CTX-1 accounted for >90% of total toxicity in this barracuda and was confirmed to be a competitive inhibitor of brevetoxin binding to voltage-sensitive sodium channels (VSSCs) with a potency two-times lower than P-CTX-1. However, FAT active on VSSCs and <900 Da were suspected to contribute to the overall toxicity.</div>
</front>
</TEI>
<affiliations><list><country><li>Australie</li>
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<tree><country name="France"><region name="Région Normandie"><name sortKey="Pottier, Ivannah" sort="Pottier, Ivannah" uniqKey="Pottier I" first="Ivannah" last="Pottier">Ivannah Pottier</name>
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<name sortKey="Vernoux, Jean Paul" sort="Vernoux, Jean Paul" uniqKey="Vernoux J" first="Jean Paul" last="Vernoux">Jean Paul Vernoux</name>
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<country name="Australie"><noRegion><name sortKey="Hamilton, Brett" sort="Hamilton, Brett" uniqKey="Hamilton B" first="Brett" last="Hamilton">Brett Hamilton</name>
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<name sortKey="Hamilton, Brett" sort="Hamilton, Brett" uniqKey="Hamilton B" first="Brett" last="Hamilton">Brett Hamilton</name>
<name sortKey="Hamilton, Brett" sort="Hamilton, Brett" uniqKey="Hamilton B" first="Brett" last="Hamilton">Brett Hamilton</name>
<name sortKey="Jones, Alun" sort="Jones, Alun" uniqKey="Jones A" first="Alun" last="Jones">Alun Jones</name>
<name sortKey="Lewis, Richard J" sort="Lewis, Richard J" uniqKey="Lewis R" first="Richard J." last="Lewis">Richard J. Lewis</name>
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Identification of slow and fast-acting toxins in a highly ciguatoxic barracuda (Sphyraena barracuda) by HPLC/MS and radiolabelled ligand binding

Identification of slow and fast-acting toxins in a highly ciguatoxic barracuda (Sphyraena barracuda) by HPLC/MS and radiolabelled ligand binding

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri